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Educerie · IB Diploma · Biology

Theme A — Topic test

Paper 2 style · 20 marks · 30 minutes

Answer all questions. The number of marks indicates the number of distinct points expected.


1. (a) Outline two properties of water that result from its polarity. [2]

(b) Explain why methane, a molecule of similar size to water, is a gas at room temperature while water is a liquid. [3]


2. (a) State the base that pairs with guanine in DNA, and the number of hydrogen bonds between them. [1]

(b) Distinguish between the structure of a prokaryotic and a eukaryotic cell. [3]

(c) Outline two pieces of evidence that mitochondria evolved from free-living prokaryotes. [2]


3. A biologist sequences a section of the same gene in four primate species and counts the number of base differences from humans.

SpeciesBase differences from human
Chimpanzee4
Gorilla9
Orangutan21
Gibbon34

(a) Deduce which species shares the most recent common ancestor with humans, using the data. [2]

(b) Suggest one limitation of using a single gene to determine evolutionary relationships. [2]


4. (a) Define the term species. [1]

(b) Explain how allopatric speciation can occur. [4]


Total: 20 marks

Command terms in this test. Question 1(b) and 4(b) say explain, so a description of what happens will not score — the mechanism or reason is required. Question 2(b) says distinguish, which must be written as a comparison, not two separate accounts. Question 3(a) says deduce, so your answer must refer to the data in the table. Question 3(b) says suggest — the answer is not in the syllabus and you are expected to reason.


Educerie · original questions written against the published IB syllabus structure for Biology Theme A, first assessment 2025. All data are our own. Last reviewed 5 September 2026.